Blastomyces dermatitidis

• Specimen: Sputum

• Stain: Wet Preparation

• Magnification: x400

• Thick-walled, broad base budding yeast

• Mother cell and daughter cell are attached, showing broad base spot. CW reagent binds cell wall and does not show internal details.

• Bright filed microscopy is helpful to display structures that are sometimes not seen by CW