Blastomyces dermatitidis

• Specimen: Sputum

• Stain: Gram stain

• Magnification: x1000

• Large, near round, thick-walled, broad base budding yeast

• Large round single cells when sitting side by side, may be confused with immature spherules of Coccidioides or detached Blastomyces.

• To separate one from the other, observe true budding, or
variable cell size including other structures. In our example, Gram smear confirmed Blastomyces