Department of Microbiology: Online Lab Manual

Virology Manual

Diagnostic Virology is performed for a variety of reasons, ranging from the diagnosis of an acute illness to the determination of asymptomatic carrier state.

The methods used to diagnose viral infections are based on the fact that many viruses produce characteristic changes in cells of the host and that most of them induce the production of infectious viruses or viral antigens in body tissues, secretions and excretions. This in turn is usually followed by the production of antibodies, which are specific for the virus and its associated antigens.

Disclaimer: These documents have been developed specifically for our institutions and may not be appropriate for implementation in other settings. This information is made available for professional education purposes only and each piece of information should be carefully evaluated before being adapted to meet the needs of other facilities or settings.

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Introduction
  Fluids and Tissues Specimens
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» Cerebral Spinal Fluid (CSF)

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» Blood / Bone Marrow For CMV Antigenemia

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» Sterile Fluids

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» Tissue/Biopsy Specimens

  Respiratory Tract Specimens
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» Broncho-Alveolar Lavage (BAL) - CMV Surveillance (PMH)

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» Throat/Nasopharyngeal/Nasal Swabs (Symptomatic)

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» ETT/Auger Suction (Infants)

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» Bronchoscopy/BAL/Sputum/Washings (Symptomatic)

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» Throat/Mouth/Washings (PMH)

  Ocular Specimens
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» Ocular Specimens

  Oro-Facial, Genital, and Skin Lesions
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» Genital/Peri-Anal/Mouth/Nose/Skin Lesions

  Gastrointestinal
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» Faeces/Rectal

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» Urine

  Appendices
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» Appendix I: Reagents/Kits

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» Appendix II: Shell Vial Procedure

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» Appendix III: Tube Culture Procedure

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» Appendix IV: Indirect Immunofluorescent Antibody (IFA) Staining for Viral Culture Confirmation

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» Appendix V: Direct Immunofluorescent Antibody (DFA) Staining

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» Appendix VI: Direct Antigen Detection From Specimens

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» Appendix VII: Hemadsorption Of Tube Culture Monolayers

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» Appendix VIII: Media

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» Appendix IX: Trypsinization and Maintenance of Monolayer Cell Cultures

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» Appendix X: Cryopreservation of Cell Cultures

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» Appendix XI: Recovery of Cryopreserved Cells

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» Appendix XII: Cryopreservation of Virus Isolates

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» Appendix XIII: Preservation of Cell Culture Monolayers

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» Appendix XIV: Quality Control Of Cell Cultures Used For Routine Virus Isolates

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» Appendix XV: Virus Isolation and Identification of Characteristics

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» Appendix XVI: Weekly Work Schedule

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» Appendix XVII: Virology Training Guide

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» Appendix XVIII: Quality Control of Monoclonal Antibodies

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» Appendix XIX: Pneumocystis carinii DFA Test

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» Appendix XX: Cytospin Preparation

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» Appendix XXI: Specimens, Cell Lines and Stain Table

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» Appendix XXII: Sputolysin Procedure

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Copyright 1999-2007 Department of Microbiology, Mount Sinai Hospital, Toronto, Canada. All rights reserved.