Department of Microbiology: Online Lab Manual

Mycology Manual

Methods used to isolate, identify, and report mycology samples.

Disclaimer: These documents have been developed specifically for our institutions and may not be appropriate for implementation in other settings. This information is made available for professional education purposes only and each piece of information should be carefully evaluated before being adapted to meet the needs of other facilities or settings.

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Title
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Lab Safety
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Daily Routine Of The Mycology Lab
QPCMI02001
Pre-analytical Procedure - Specimen Collection
QPCMI06003 Specimen Processing Procedure
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Isolation and Identification
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Reporting
  Staining Methods
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» Acid-Fast Stain For Nocardia (Modified Kinyoun)

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» Calcofluor White Stain

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» Fungi-Fluor™ Stain

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» Gomori Methenamine Silver (GMS) AND Periodic Acid Schiff (PAS) Fungal Stains

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» India Ink

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» Lactophenol Aniline Blue (LPAB)

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Isolator 10 Blood Culture System For Dimorphic Fungi
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References
  Appendices
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» Appendix I: Conversion (Converting Mycelial Phase of Dimorphic Mould to a Yeast Phase)

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» Appendix II: Cornmeal Tween-80/Oxgall Agar

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» Appendix III: Determining Cycloheximide Resistance Of An Isolate

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» Appendix IV: Fluorescent Microscope (Instructions)

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» Appendix V: Germ Tube Test

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» Appendix VI: Slide Culture

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» Appendix VII: Stock Cultures -Water Culture Technique

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» Appendix VIII: RAPI 20 CAUX - Yeast Identification System

  Appendix X: Media / Reagents
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» Blood Egg Albumin Agar (BEAA)

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» Brain Heart Infusion Agar

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» Cornmeal Tween 80 Agar (Oxoid)

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» Esculin Base Medium (EBM) pH 7.1

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» Inhibitory Mold Agar (IMA)

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» Lactophenol Aniline Blue Stain (LPAB)

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» Mycosel Agar

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» Oxgall Agar

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» Potato Dextrose Agar (PDA)

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» Sodium Pyruvate Agar (NPA) for Nocardia

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» Sabouraud Agar Modified (DIFCO)

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» Sabouraud Gentamicin Agar Slopes

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» Urea Agar Slopes

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» 10% Potassium Hydroxide

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MI\MYC Download entire Mycology Manual in PDF format
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© Copyright 1999-2007 Department of Microbiology, Mount Sinai Hospital, Toronto, Canada. All rights reserved.